Journal: Journal of Innate Immunity
Article Title: Single-Cell RNA Sequencing in Pediatric Sepsis: γδ T Cell Exhibits a Differentiation to γδT17 Subtype along with Significantly Enhanced Cell Communication with Neutrophils
doi: 10.1159/000547934
Figure Lengend Snippet: Comparison of cell-cell communication analysis of γδ T cells in single-cell mRNA sequencing between the pediatric sepsis (sepsis) group and HC group. a Comparison of resistin signaling. b Comparison of interactions between cell surface receptors and cell ligands in resistin signaling. c Comparison of mRNA expression levels of cell surface receptors and cell ligand genes in all cell subtypes during resistin signaling. d Comparison of RNA expression levels of CAP1 gene in γδ T cells between sepsis ( n = 5) and HC samples ( n = 5). e The proportion of IL-17A-positive γδ T cells was detected by flow cytometry in resistin stimulation ( n = 5) and control ( n = 5). f Comparison of the proportion of IL-17A-positive γδ T cells between resistin stimulation ( n = 6) and control ( n = 6) (* p < 0.05).
Article Snippet: For IL-17A induction, PBMCs were cultured with resistin (100 ng/mL, MCE) and brefeldin A (10 μg/mL, Sigma-Aldrich) for 4 h, followed by flow cytometry.
Techniques: Comparison, Sequencing, Expressing, RNA Expression, Flow Cytometry, Control